The isolation of asparagine from an enzymic digest of edestin.
نویسنده
چکیده
منابع مشابه
Lxxxiv. the Hofmann Degradation of Glutamine Residues in Gliadin by Richard Laurence Millington Synge
THE question of the mode of linkage of the ammonia liberated in the hydrolysis of proteins was reviewed by Damodaran [1932] in his paper describing the isolation of asparagine from an enzymic digest of edestin. This work provided the first direct evidence for the long-standing hypothesis that the ammonia so liberated has its origin in the hydrolysis of glutamine and asparagine residues. Shortly...
متن کاملIsolation and Characterization of Edestin from Cheungsam Hempseed
Edestin, a major hempseed storage protein from Cheungsam, was isolated to apparent homogeneity by acid precipitation and gel filtration chromatography. The native molecular weight of purified edestin was approximately 300 kDa by Sephacryl S-300 gel filtration. Upon adding 2mercaptoethanol, the isolated edestin of 56.7 kDa on the non-reduced sodium dodecyl sulfate polyacrylamide gel was converte...
متن کاملScreening for Type II L-Asparaginases: Lessons from the Genus Halomonas
Among the two types of bacterial L-asparaginases, only type II enzymes have been used inthe treatment of acute lymphoblastic leukemia owing to their higher affinity for L-asparagine.However, current screening media used for the isolation of L-asparaginase-producingmicroorganisms do not discriminate between the two types of L-asparaginase. During anoptimization study conducted to increase L-aspa...
متن کاملScreening for Type II L-Asparaginases: Lessons from the Genus Halomonas
Among the two types of bacterial L-asparaginases, only type II enzymes have been used inthe treatment of acute lymphoblastic leukemia owing to their higher affinity for L-asparagine.However, current screening media used for the isolation of L-asparaginase-producingmicroorganisms do not discriminate between the two types of L-asparaginase. During anoptimization study conducted to increase L-aspa...
متن کاملChanges of glutamine and asparagine content in cucumber seedlings in response to nitrate stress
Nitrogen fertilizer application rates in intensive agricultural systems have increased dramatically in recent years, especially in protected vegetable production systems. This excessive use of nitrogen fertilizer has resulted in soil secondary salinity, which has become a significant environmental stress for crops such as cucumber, in the protected farmlands. It is thus necessary to illuminate ...
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ورودعنوان ژورنال:
- The Biochemical journal
دوره 26 1 شماره
صفحات -
تاریخ انتشار 2005